--- title: "Publication-Ready Output" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{Publication-Ready Output} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r setup, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>", fig.width = 10, fig.height = 7 ) ``` ## Color-Blind Friendly Palettes Rclade defaults to the `viridis` palette, which is: - Color-blind friendly - Grayscale friendly - Perceptually uniform ```{r palette} library(Rclade) data(example_tree) # Default viridis palette p <- plot_timetree(example_tree, rank = "phylum", taxonomy_format = "GTDB", add_timescale = FALSE) print(p) ``` ## Custom Color Mapping ```{r custom_colors} # Custom color mapping for specific groups p <- plot_timetree(example_tree, rank = "phylum", taxonomy_format = "GTDB", add_timescale = FALSE, color_mapping = c("Proteobacteria" = "#E41A1C", "Firmicutes" = "#377EB8")) print(p) ``` ## Legend Placement ```{r legend} # Inside the plot (default) p <- plot_timetree(example_tree, rank = "phylum", taxonomy_format = "GTDB", add_timescale = FALSE, legend_position = c(0.05, 0.85)) # Standard positions p <- plot_timetree(example_tree, rank = "phylum", taxonomy_format = "GTDB", add_timescale = FALSE, legend_position = "right") ``` ## Clade Labels ```{r clade_labels} p <- plot_timetree(example_tree, rank = "phylum", taxonomy_format = "GTDB", add_timescale = FALSE, show_clade_label = TRUE) print(p) ``` ## Taxonomy Quality Report Before finalizing your figure, verify label parsing quality: ```{r quality} summarize_taxonomy_quality(example_tree$tip.label, format = "GTDB") ``` ## Batch Processing Process multiple tree files at once: ```{r batch, eval = FALSE} batch_plot(input_dir = "trees/", output_dir = "figures/", pattern = "*.tre", rank = "phylum", taxonomy_format = "GTDB") ``` ## Reproducibility ```{r reproducibility, eval = FALSE} save_session_info("session_info.txt") ``` ## References & Acknowledgments Rclade builds on the **ggtree** and **deeptime** R packages. If you use Rclade in published research, please cite Rclade along with these key dependencies: - Yu G, Smith DK, Zhu H, Guan Y, Lam TT-Y (2017). "ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data." *Methods in Ecology and Evolution*, 8(1), 28-36. doi:10.1111/2041-210X.12628 - Gearty W (2025). "deeptime: an R package that facilitates highly customizable and reproducible visualizations of data over geological time intervals." *Big Earth Data*. doi:10.1080/20964471.2025.2537516 The geological timescale data is based on the ICS International Chronostratigraphic Chart 2023/02 (https://stratigraphy.org/chart/).